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74092

Sigma-Aldrich

Sodium phosphate monobasic solution

BioUltra, 5 M in H2O

Synonym(s):

Monosodium phosphate solution, Sodium dihydrogen phosphate, Sodium dihydrogen orthophosphate,Sodium orthophosphate monobasic, Dihydrogen monosodium phosphate, Dihydrogen sodium phosphate

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About This Item

Empirical Formula (Hill Notation):
H2NaO4P
CAS Number:
7558-80-7
Molecular Weight:
119.98
Beilstein:
3903772
MDL number:
MFCD00003527
UNSPSC Code:
12161700
PubChem Substance ID:
57652500
NACRES:
NA.25

product line

BioUltra

Quality Level

200

form

liquid

concentration

5 M in H2O

impurities

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

3.0-4.5 (25 °C, 5 M in H2O)

solubility

H2O: 5 M, clear, colorless

density

1.40 g/mL at 20 °C

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025
λ: 280 nm Amax: ≤0.02

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OP(O)([O-])=O

InChI

1S/Na.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

AJPJDKMHJJGVTQ-UHFFFAOYSA-M

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General description

Sodium phosphate monobasic serves as a source of phosphate ions. The use of sodium phosphate monobasic to correct severe hypophosphatemia conditions has been reviewed.

Application

Sodium phosphate monobasic solution has been used as a component of aCSF (artificial cerebrospinal fluid) stock solution for use in the patch-seq analysis of small interneurons. It has also been used as a supplement in the preparation of crude extract of cells for decentralizing cell-free RNA sensing.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts
Arce A, et al.
Frontiers in Bioengineering and Biotechnology (2021)
Phosphorus availability from inorganic phosphorus sources in yellowtail (Seriola quinqueradiata Temminck and Schlegel)
Sarker PK, et al.
Aquaculture (Amsterdam, Netherlands) (2009)
Marcela Lipovsek et al.
STAR protocols, 1(3), 100146-100146 (2020-12-31)
Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands
Samuel Adler et al.
Gastrointestinal endoscopy, 76(6), 1170-1174 (2012-10-03)
Colon capsule endoscopy (CCE) is a noninvasive technique for the detection of colorectal lesions. However, for CCE to be offered as an out-of-clinic procedure, the system needs to automatically alert the patient when to ingest the laxative (booster). We tested
Charles W Kimbrough et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(20), 4576-4585 (2015-07-01)
pH-low insertion peptides (pHLIP) can serve as a targeting moiety that enables pH-sensitive probes to detect solid tumors. Using these probes in conjunction with multispectral optoacoustic tomography (MSOT) is a promising approach to improve imaging for pancreatic cancer. A pH-sensitive
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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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