32855

Eosin Methylene blue

according to May-Grünwald

• Colour Index Number: 881/1038
• Colour Index Number: 45380/52015
• MDL number: MFCD00005038
• UNSPSC Code: 12171500
• PubChem Substance ID: 329754361
• NACRES: MA.02

Properties

• form: powder or crystals
• Quality Level: 200
• quality: according to May-Grünwald
• εmax: ≥1000 at 635-655 nm in methanol; ≥650 at 512-532 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
• InChI key: NXBZXYJXNIFCRJ-UHFFFAOYSA-M

Description

General description

Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.

Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Application

Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves