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228842

Sigma-Aldrich

Ethyl Violet

cationic triarylmethane dye

Synonym(s):

Basic violet 4, Ethyl purple 6B

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About This Item

Linear Formula:
C31H42N3Cl
CAS Number:
2390-59-2
Molecular Weight:
492.14
Colour Index Number:
42600
Beilstein:
4117566
EC Number:
219-231-5
MDL number:
MFCD00011765
UNSPSC Code:
12171500
PubChem Substance ID:
24853715
NACRES:
NA.47

Quality Level

200

form

powder

solubility

water: 1 mg/mL

λmax

595 nm

ε (extinction coefficient)

≥12000 at 250-256 nm in water at 0.003 mg/mL
≥17000 at 304-310 nm in water at 0.003 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCN(CC)c1ccc(cc1)C(\c2ccc(cc2)N(CC)CC)=C3/C=C\C(C=C3)=[N+](\CC)CC

InChI

1S/C31H42N3.ClH/c1-7-32(8-2)28-19-13-25(14-20-28)31(26-15-21-29(22-16-26)33(9-3)10-4)27-17-23-30(24-18-27)34(11-5)12-6;/h13-24H,7-12H2,1-6H3;1H/q+1;/p-1

InChI key

JVICFMRAVNKDOE-UHFFFAOYSA-M

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General description

Ethyl Violet is an olive green colored powder and belongs to the class of triarylmethane dyes. It changes its color from yellow (pH 0.0) to blue (3.5).

Biochem/physiol Actions

Ethyl violet is a cationic triarylmethane dye that is the ethyl homolog of crystal violet. It is a general histological and pathological stain. Ethyl violet has been used to stain pancreatic tissue and elastin, in the detection of gram negative bacteria, and as a reagent for the extractive-spectrophotometric determination of anionic surfactants. Ethyl violet induces mitochondrial permeability. It has been used in the extraction and spectrophotometric determination of copper. Along with the anionic dye zincon, ethyl violet is used in counterion dye of proteins in SDS-polyacrylamide gel electrophoresis.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR9526
0000233367
MKCC4353
MKBV4458V
MKBV1374V

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Ethyl violet. A selective dye for the isolation of gram-negative bacteria.
Litsky, W., et al.
Biotechnic & Histochemistry, 27, 229-232 (1952)
Mohd Talib Latif et al.
Environmental science & technology, 38(24), 6501-6506 (2005-01-27)
The concentration and characteristics of anionic and cationic aerosol surfactants at a range of locations were determined as methylene blue active substances (MBAS) and ethyl violet active substances (EVAS) for anionic surfactants and as disulfine blue active substances (DBAS) for
W T Tsai et al.
Journal of colloid and interface science, 289(2), 333-338 (2005-06-01)
The adsorption of basic dye (i.e., ethyl violet or basic violet 4) from aqueous solution onto the regenerated spent bleaching earth (RSBE) was carried out by varying the process parameters such as initial concentration, pH, and temperature. As analytical comparisons
Wei-Tao Cong et al.
Methods in molecular biology (Clifton, N.J.), 869, 497-509 (2012-05-16)
A fast and matrix-assisted laser desorption/ionization mass spectrometry compatible protein staining method in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis is described. It is based on the counterion dye staining method that employs oppositely charged two dyes, zincon and
Solvent extraction of copper as its ion association complex with ethyl violet and its application to the spectrophotometric determination of copper.
Yamamoto, K. and Motomizu, S.
Analyst, 112, 1011-1014 (1987)
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