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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Specific activity:
>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
Biological source:
enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
Concentration:
1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
biological source
enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
sterility
non-sterile
form
lyophilized
collagenase activity
>0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)
specific activity
>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
packaging
pkg of 100 mg (10269638001), pkg of 500 mg (11097113001)
manufacturer/tradename
Roche
concentration
1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
technique(s)
tissue processing: suitable
color
light yellow to brown
optimum pH
7.0-8.0
solubility
water: soluble
UniProt accession no.
application(s)
life science and biopharma
sample preparation
foreign activity
proteolytic activities <0.1 U/mg (hemoglobin as the substrate)
storage temp.
2-8°C
General description
Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.
Application
Collagenase/Dispase® is used for the preparation of cells from a wide variety of different tissues and organs.
Biochem/physiol Actions
Collagenase degrades native collagen. Dispase® is a nonspecific protease.
Preparation Note
Activator: Ca2+
Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)
Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS.
Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)
Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)
Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS.
Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca2+/Mg2+-free).
Store dry
Other Notes
Collagenase from Vibrio alginolyticus, Dispase® from Bacillus polymyxa
EC 3.4.24.4 & 3.4.24.3
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.
Legal Information
Dispase is a registered trademark of Godo Shusei Co., Ltd.
signalword
Danger
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
hcodes
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10269638001 | 04061838250520 |
| 11097113001 | 04061838250681 |
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