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Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate

clone 4G10®, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody product type

primary antibodies

clone

4G10®, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr)

Gene Information

human ... PID1(55022)

General description

Anti-phosphotyrosine antibody (05-321), cross-linked to fluorescein isothiocyanate (FITC).

Specificity

Recognizes tyrosine-phosphorylated proteins from all species.

Immunogen

Phosphotyramine-KLH.

Application

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, FITC conjugate validated for use in IC.
For use in applications in which a FITC conjugate is advantageous, such as ICC.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Quality

routinely evaluated in immunocytochemistry to detect tyrosine phosphorylated proteins in EGF-stimulated human A431 cells

Physical form

PBS containing 0.05% sodium azide
Protein G Purified

Storage and Stability

Stable for 9 months at 4°C

Analysis Note

Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B Josh Lane et al.
PLoS pathogens, 4(3), e1000014-e1000014 (2008-04-04)
Chlamydia trachomatis attachment to cells induces the secretion of the elementary body-associated protein TARP (Translocated Actin Recruiting Protein). TARP crosses the plasma membrane where it is immediately phosphorylated at tyrosine residues by unknown host kinases. The Rac GTPase is also
David Vandael et al.
Nature protocols, 16(6), 2947-2967 (2021-05-16)
Rigorous investigation of synaptic transmission requires analysis of unitary synaptic events by simultaneous recording from presynaptic terminals and postsynaptic target neurons. However, this has been achieved at only a limited number of model synapses, including the squid giant synapse and
Marina Guillén-Yunta et al.
Fluids and barriers of the CNS, 20(1), 79-79 (2023-11-04)
The monocarboxylate transporter 8 (MCT8) plays a vital role in maintaining brain thyroid hormone homeostasis. This transmembrane transporter is expressed at the brain barriers, as the blood-brain barrier (BBB), and in neural cells, being the sole known thyroid hormone-specific transporter
Xiaomin Zhang et al.
Neuron, 107(6), 1212-1225 (2020-08-09)
Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials
Neetu Gupta et al.
Molecular biology of the cell, 14(2), 432-444 (2003-02-18)
Recent biochemical evidence indicates that an early event in signal transduction by the B-cell antigen receptor (BCR) is its translocation to specialized membrane subdomains known as lipid rafts. We have taken a microscopic approach to image lipid rafts and early

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