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Key Documents

1.04137

Sigma-Aldrich

Crystal violet (C.I. 42555)

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About This Item

Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
MDL number:
UNSPSC Code:
41116130
NACRES:
NA.21

Pricing and availability is not currently available.

form

solid

Quality Level

potency

420 mg/kg LD50, oral (Rat)

composition

Dye, ≥88% spectrophotometric assay

pH

2.5-3.5 (20 °C, 10 g/L in H2O)

mp

189-194 °C

solubility

10 g/L

density

1.19 g/L at 20 °C

bulk density

220‑400 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

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General description

Crystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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