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EZHS100B

Millipore

Human S100B ELISA Kit

quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma

Synonym(s):

Protein S100B, S-100 protein beta chain, S100 calcium-binding protein B

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Pricing and availability is not currently available.

Product Name

Human S100B ELISA, EZHS100B-33K, This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.

Quality Level

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

assay range

accuracy: 93%
(CSF)

accuracy: 94%
(serum)

sensitivity: 2.7 pg/mL
standard curve range: 2.7-2000 pg/mL
inter-assay cv: <4%
intra-assay cv: <4%

technique(s)

ELISA: suitable

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

application(s)

research use

detection method

colorimetric (450nm)

shipped in

wet ice

storage temp.

2-8°C

General description

EMD Millipore’s Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum and plasma. This kit has not been tested for cell culture supernatants.
S100B is a calcium-binding protein produced mainly by astrocytes in the central nervous system and has been implicated in the development and maintenance of the nervous system. Although the mechanism of S100B secretion is unknown, it appears to be affected by oxidative stress. At nanomolar concentrations (normal levels), S100B is able to protect neurons against glutamate toxicity. Glial damage or astrocytic reactions to neural injury (reactive astrogliosis) causes an increase in S100B to micromolar concentrations. High levels of S100B will further increase neuronal damage. High levels of S100B are considered a biomarker that could indicate damage or dysfunction of CNS.

Specificity

The antibody pair used in this assay measures Human S100B and has no cross-reactivity with S100A1, S100A6 and S100A13.

This assay detects S100B in multiple species, including: bovine, hamster, horse, monkey, mouse, pig, rabbit, and rat.

Application

Research Category
Neuroscience
This is a same day assay
Used to detect/quantify: S100B

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alison Crichton et al.
Journal of neurotrauma, 38(8), 1151-1163 (2019-12-28)
Despite many children experiencing fatigue after childhood brain injury, little is known about the predictors of this complaint. To date, traditional indices of traumatic brain injury (TBI) severity have not predicted reliably persisting fatigue (up to three years post-injury). This

Questions

1–2 of 2 Questions  
  1. Is the EZHS100B-33K ELISA assay compatible with human saliva samples?

    1 answer
    1. The kits have not been validated for use with saliva; however, some guidelines are available for processing saliva samples:


      1. Add protease inhibitor cocktail (Product P2714) at a 1:500 ratio to the saliva.
      2. Centrifuge the mixture at 10,000 rpm for 10 minutes.
      3. Dilute the supernatant in a 1:2 ratio with Assay Buffer before to setting up the assay.
      4. Run assay with Assay Buffer as the matrix for the standard curve.
      5. Multiply by 2 for the final result.


      It should be noted that there are no references available for the S100B ELISA kit being used with saliva samples.

      Helpful?

  2. Which antibodies have been used for the Human S100B ELISA kit (EZHS100B-33K)?

    1 answer
    1. The origins of the antibodies utilized in the kit are considered proprietary. Nevertheless, some information about the antibodies can be shared. The capture antibody is a monoclonal mouse anti-S100B, with bovine brain S100B used as the immunogen. The detection antibody is a polyclonal rabbit anti-S100, and the immunogen for this is also sourced from bovine brain S100.

      Helpful?

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