Reverse the orientation of the column. Flush for a few minutes with a 20% aqueous solution of the organic used in the analysis to remove buffer salts. Flush for about 30 mins with 100% of the organic used in the analysis. The column can be stored with this organic solution. Before reusing the column, flush again for a few minutes with 20% organic to prevent precipitation of buffer salts.
Z226262
Nucleosil® CN HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
Synonym(s):
Cyanopropyl HPLC Column
About This Item
Recommended Products
Agency
suitable for USP L10
Quality Level
product line
Nucleosil®
feature
endcapped: no
manufacturer/tradename
Nucleosil®
extent of labeling
5% Carbon loading
parameter
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
350 m2/g
matrix
cyano
matrix active group
cyano phase
particle size
5 μm
pore size
100 Å
separation technique
hydrophilic interaction (HILIC)
normal phase
reversed phase
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