G004415
Improved HPLC Selectivity of Discovery® BIO Wide Pore RP Phases
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 μm particles vs. competitive wide pore C18 column of same dimensions (568222-U)
mobile phase: [A] 0.1% TFA in 80:20 water:acetonitrile; [B] 0.1% TFA in 66:34 water:acetonitrile
Gradient of 0 to 100% B in 14 min. after 1 min. delay
gradient: 0 to 100% B in 14 min. after 1 min. delay
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 220 nm
injection: 10 μL
sample: peptide mixture (Sigma P2693), ~0.25 μg each peptide in 0.1% TFA
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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