ZRB2397

Anti-SIPA1 Antibody, clone 1D5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Signal-induced proliferation-associated protein 1 (UniProt: Q96FS4, also known as Sipa-1, GTPase-activating protein Spa-1, p130 SPA-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D5, recombinant monoclonal
• description: 1D5 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 112.15 kDa; observed mol wt ~110 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_006738
• UniProt accession no.: Q96FS4
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SIPA1(6494)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Signal-induced proliferation-associated protein 1 (SIPA1).

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Signal-induced proliferation-associated protein 1 (SIPA1).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SIPA1 in Mouse spleen tissue lysate.

Tested Applications.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SIPA1 in Human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound SIPA1 peptide with a KD of 2.6 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SIPA1 in Jurkat and THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Signal-induced proliferation-associated protein 1 (UniProt: Q96FS4; also known as Sipa-1, GTPase-activating protein Spa-1, p130 SPA-1) is encoded by the SIPA1 (also known as SPA1) gene (Gene ID: 6494) in human. SIPA1 is a GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 that negatively regulates Rap via hydrolysis of active GTP-Rap to inactive GDP-Rap. It is mostly localized in the perinuclear membranous region, co-locating with Rap1 and Rap2. It is expressed in fetal and adult tissues and is abundantly expressed in thymus, spleen, peripheral blood lymphocytes, and spinal cord. Three major domains are identified in SIPA1: Rap-GAP domain (aa 321-539); PDZ domain (aa 687-763) and a coiled coil domain (aa 972-1034). SPA-1 is involved in the metastasis of various solid tumors and is indicative of a poor prognosis. In MDA-MB-231 cells SIPA1 is mainly localized in the nucleus and knockdown of SIPA1 gene reduces adhesion, migration, and invasion in these cells and reduces their metastatic capacity. However, it promotes these cells to proliferate. Nuclear SIPA1 is shown to interact with and activate integrin β1 promoter in breast cancer cells.  This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, C., et al. (2020). Mol. Clin. Oncol. 13(4); 32; Kurachi, H., et al. (1997). J. Biol. Chem. 272(44); 2881-28088).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable