ZRB1674

Anti-RHOT1/MIRO-1 Antibody, clone 1F4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Mitochondrial Rho GTPase 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F4, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 70.78 kDa; observed mol wt ~70 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_060777
• UniProt accession no.: Q8IXI2
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RHOT1(55288)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F4 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mitochondrial Rho GTPase 1 (MIRO-1). It targets an epitope within 22 amino acids from the N-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, cytoplasmic domain of human Mitochondrial Rho GTPase 1 (MIRO-1).

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RHOT1/MIRO-1 in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RHOT1/MIRO-1 in human skeletal muscle and NIH3T3 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RHOT1/MIRO-1 in A549 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RHOT1/MIRO-1 in human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Mitochondrial Rho GTPase 1 (UniProt: Q8IXI2; also known as MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1) is encoded by the RHOT1 (also known as ARTH1) gene (Gene ID: 55288) in human. MIRO-1 is a single-pass type IV membrane protein found on the outer mitochondrial membrane. It contains a large cytoplasmic domain (aa 1-592), a transmembrane domain (aa 593-615), and a short mitochondrial intermembrane domain (aa 616-618). It contains structurally distinct N-terminal and C-terminal GTP-binding motif with a linker region (MiroS) connecting two EF-hand domains (aa 184-219 and 304-339) to the C-terminal GTPase domain. It also contains two ligand-mimicking -helices (LM1 and LM2) that connect each canonical EF hand to a non-canonical hidden EF-hand domain. The calcium-binding amino acids (aa 197-208 and 317-328) of MIRO-1 are exposed to the cytosol via a helix-loop-helix-motif in both EF hands, facilitating a conformational change of the protein upon binding to calcium. MIRO-1 is involved in mitochondrial trafficking and control of anterograde transport of mitochondria and their subcellular distribution. It undergoes ubiquitination by parkin that leads to its degradation and enhancement of mitophagy. Proteins encoded by several Parkinson s disease linked genes are reported to physically interact with MIRO-1 and modify its function and contribute to the dysregulation of neuronal integrity. Neuron-specific loss of MIRO-1 in a murine model is shown to cause depletion of mitochondria from corticospinal tract axons and progressive neurological deficits that mirrors human upper motor neuron disease. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Grossmann, D., et al. (2020). Front. Neurol. 11; 587; Nguyen, TT., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(35); E3631-E3640).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable