TMB is a chromogenic substrate for horseradish peroxidase (HRP). The complete formulation of this product is considered proprietary. However, the color change to blue/purple is due to the oxidation of the TMB substrate, which is catalyzed by peroxidase.
T9455
TMB Enhanced One Component HRP Membrane Substrate
Synonym(s):
HRP substrate, TMB, detection reagent, detection substrate
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.
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General description
TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.
Application
Caution
Stable at least one year at 2-8 °C.
Physical form
Ready-to-use.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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The webpage states it produces a deep blue/purple product with HRP, and the unreacted substrate should be pale yellow to pink. Our professor wants to know the components causing this purple color. Could you please look into this for us? Thank you.
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