The structure of the material after purification is not assessed. While the native folding state cannot be guaranteed, the final purified flagellan can bind human and mouse TLR5 and activate TLR5 in cell culture.
SRP8029
Flagellin from Salmonella typhimurium
>95% (SDS-PAGE)
Synonym(s):
FliC, Intelectin-1
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About This Item
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biological source
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Assay
>95% (SDS-PAGE)
form
lyophilized
mol wt
~50 kDa by SDS-PAGE
packaging
pkg of 10 μg
impurities
<0.01 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ... fliC (STM1959)
General description
FliC (flagellin) is the subunit protein which polymerizes to form the filaments of bacterial flagella. The protein has four-domains (D0, D1, D2, D3).[1]
The encoded flagellin filament contains 55 kDa monomers.[2]
Biochem/physiol Actions
FliC (flagellin) can activate the innate immune system by TLR5 (toll like receptor 5) and is an important proinflammatory molecule.[1][3] FliC also induces the production of antibodies and is identified as an antigen by CD4+ T cells.[4]
It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α.[5]
Physical form
Lyophilized from PBS.
Reconstitution
Reconstitute with 100 μL deionized water.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways.
Zeng H, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(1), G96-G108 (2006)
FliC-specific CD4+ T cell responses are restricted by bacterial regulation of antigen expression.
Cummings LA
Journal of Immunology, 174, 7929-7938 (2005)
Mucosal administration of flagellin induces innate immunity in the mouse lung.
Honko A N and Mizel S B
Infection and Immunity, 72(11), 6676-6679 (2004)
In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted.
Cummings LA
Molecular Microbiology, 61, 795-809 (2006)
Laís Rossi Perazza et al.
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Hello! I wondered if this product is expressed from the host in its native folded state? I am interested in obtaining native folded flagellin... thanks for any insights you can provide in this matter!
1 answer-
Helpful?
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Good morning, I would like to have the sequence of this protein and the host where it was produced. Thank yo and best regards
1 answer-
This is native Flagellin purified from Salmonella typhimurium. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P06179/entryHelpful?
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