immunoprecipitation (IP): 5-10 μg using lysates of human HEK-293T cells. indirect immunofluorescence: 2.5-5.0 μg/mL using HEK-293T cells overexpressing human Importin 5. western blot: 2-4 μg/mL using whole extracts of human Jurkat cells.
Importin 5 (IPO5) is encoded by the gene mapped to human chromosome 13q32.2. The encoded protein belongs to the importin β family. It is primarily expressed in cytoplasm, specifically in the nuclear pore complex.
Application
Anti-Importin 5 (N-terminal) antibody produced in rabbit has been used in western blotting, immunoprecipitation and immunofluorescence.
Biochem/physiol Actions
Importin 5 (IPO5) in association with nucleoside triphosphates and the small GTP binding protein Ran, facilitates migration of nuclear localization signal (NLS)-containing cargo from cytoplasm to nucleus. IPO5 also plays a vital role in apolipoprotein A-1 excretion and also helps in transport of lipids from peripheral tissues via reverse cholesterol transport pathway. Additionally, the encoded protein facilitates transport of ribosomal proteins and core histones into the nucleus. Mutation in the gene is associated with the development of schizophrenia.
Importin 5 (also known as IPO5, karyopherin β-3, importin subunit β-3, KPNB3 and RANBP5) has been identified as an importin for histones and proteins L23a, S7, and L5, and also to the PB1 subunit of the influenza A virus RNA-dependent RNA polymerase.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Genome-Wide Association Study of Peripheral Arterial Disease in a Japanese Population.
Matsukura M
PLoS ONE, 10 (2015)
The full-length interleukin-33 (FLIL33)--importin-5 interaction does not regulate FLIL33's nuclear localization but controls its intracellular degradation.
Clerman A
The Journal of Biological Chemistry (2017)
Characterization of the interaction between the influenza A virus polymerase subunit PB1 and the host nuclear import factor Ran-binding protein 5
Hutchinson EC, et al.
The Journal of General Virology, 92(8), 1859-1869 (2011)
Genetic and functional study of the IPO5 gene in schizophrenia.
The Journal of biological chemistry, 292(52), 21653-21661 (2017-11-12)
Human mature IL-33 is a member of the IL-1 family and a potent regulator of immunity through its pro-T helper cell 2 activity. Its precursor form, full-length interleukin-33 (FLIL33), is an intranuclear protein in many cell types, including fibroblasts, and
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