SAB2702380

Monoclonal Anti-HEC1 antibody produced in mouse

clone 9G3.23, affinity isolated antibody

• Synonym(s): HEC, HEC1, HsHec1, KNTC2
• UNSPSC Code: 12352203
• NACRES: NA.41
• conjugate: unconjugated
• application: FACS; ICC; IF; IHC; IP; PLA; WB
• clone: 9G3.23, monoclonal
• species reactivity: hamster, kangaroo rat, human
• citations: 1
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; proximity ligation assay: suitable; western blot: suitable

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: 9G3.23, monoclonal
• form: buffered aqueous solution
• mol wt: 80kDa
• species reactivity: hamster, kangaroo rat, human
• concentration: 1mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; proximity ligation assay: suitable; western blot: suitable
• isotype: IgG2a
• UniProt accession no.: O14777
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NDC80(10403)

Description

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

Application

Suggested starting dilutions are as follows: FACS: Assay-dependent dilution, ICC/IF: 1:100-1:1000, IHC: Assay-dependent dilution, IP: Assay-dependent dilution, WB: 1:500-1:3000, PLA: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Protein G Affinity Purified

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable