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S5174

Sigma-Aldrich

SPARC from murine parietal yolk sac (PYS-2) cells

~80% (SDS-PAGE), lyophilized powder

Synonym(s):

BM-40, Osteonectin, Secreted protein acidic and rich in cysteine

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About This Item

MDL number:
MFCD00213018
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

mouse (PYS-2 cells)

Assay

~80% (SDS-PAGE)

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

surface coverage

4‑40 μg/cm2

UniProt accession no.

P07214

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Sparc(20692)

Application

SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.

Biochem/physiol Actions

SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.

Components

SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.

Caution

The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.

Preparation Note

This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Sage et al.
The Journal of cell biology, 109(1), 341-356 (1989-07-01)
SPARC (Secreted Protein Acidic and Rich in Cysteine) is a Ca+2-binding glycoprotein that is differentially associated with morphogenesis, remodeling, cellular migration, and proliferation. We show here that exogenous SPARC, added to cells in culture, was associated with profound changes in
G Chen et al.
British journal of cancer, 102(1), 188-195 (2009-11-19)
Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell-cell and cell-matrix interactions. In cancer, SPARC can be not only associated with a highly aggressive phenotype, but also acts as a tumour suppressor. The aim of this
T F Lane et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8(2), 163-173 (1994-02-01)
An extracellular matrix-associated glycoprotein expressed in a variety of tissues during embryogenesis and repair, SPARC contains modular domains that can function independently to bind cells and matrix components. Because SPARC can selectively disrupt cellular contacts with matrix and thereby effect
Clément Faye et al.
The Journal of biological chemistry, 284(33), 22041-22047 (2009-06-23)
Endostatin is a C-terminal proteolytic fragment of collagen XVIII that is localized in vascular basement membrane zones in various organs. It binds to heparin/heparan sulfate and to a number of proteins, but its molecular mechanisms of action are not fully

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