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RAB0334

Sigma-Aldrich

Mouse Leptin ELISA Kit

for serum, plasma, cell culture supernatant

Synonym(s):

Leptin, Obese protein, Obesity factor, OB

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Lep(16846)

General description

The Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Leptin in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse Leptin

Application

For research use only. Not for use in diagnostic procedures.

Mouse Leptin ELISA Kit has been used to measure plasma leptin concentration.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse Leptin ELISA kit has been used to measure serum leptin concentration in adipose tissues of mice.

Biochem/physiol Actions

Leptin is a hormone secreted by adipocytes. It is majorly involved in regulation of food intake and energy balance. Variation in the leptin promoter alters serum leptin and HDLC concentration, which might lead to development of obesity. Increased concentration of serum leptin causes arterial stiffness in coronary artery disease (CAD) patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Redistribution of tissue zinc pools during lactation and dyshomeostasis during marginal zinc deficiency in mice.
McCormick NH
Journal of Trace Elements in Medicine and Biology null
Janaka Wickramasinghe et al.
Animals : an open access journal from MDPI, 12(12) (2022-06-25)
The study objective was to examine the effects of supplementing Gln and BCAA on the SFI and ADG of weaning dairy calves. Holstein heifer calves (11 calves /treatment) at 35 d of age were assigned to: (1) no amino acids
Nicholas H McCormick et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 29, 170-175 (2014-06-30)
Zinc (Zn) requirements are increased during lactation. Increased demand is partially met through increased Zn absorption from the diet. It is estimated that 60-80% of women of reproductive age are at risk for Zn deficiency due to low intake of
Leptin promoter variant G2548A is associated with serum leptin and HDL-C levels in a case control observational study in association with obesity in a Pakistani cohort.
Shabana and Hasnain S
Journal of Biosciences null
Association of serum leptin levels with central arterial stiffness in coronary artery disease patients.
Tsai JP
BMC Cardiovascular Disorders null
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