PIR01

Inhibitor Resistant Genotyping PCR ReadyMix^™, Low ROX^™

with Low ROX for ABI and Stratagene instruments

• Synonym(s): Inhibitor Resistant Genotyping PCR ReadyMix^™
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• feature: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart
• storage condition: protect from light
• technique(s): qPCR: suitable
• color: light blue
• input: crude DNA
• detection method: probe-based
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Inhibitor Resistant Genotyping PCR Readymix^™ is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hotstart allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:

• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Applied Biosystems ViiA 7
• Stratagene Mx3000P^®
• Stratagene Mx3005P^®
• Stratagene Mx4000^™

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required
• Compatible with both fast and conventional PCR cycling protocols

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.

250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable