P4600

ReadyMix^™ Taq PCR Reaction Mix

with MgCl₂

• Synonym(s): PCR master mix, Taq PCR master mix
• MDL number: MFCD01635810
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 100 reactions
• feature: dNTPs included; hotstart: no
• concentration: 1.5 units/reaction (50 μL reaction volume)
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

ReadyMix^™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Application

ReadyMix^™ Taq PCR Reaction Mix has been used:

• in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers
• in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis
• in the molecular identification of yeasts
• for routine PCR amplifications

Features and Benefits

• Amplifies targets up to 7 kb in length

• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

• ReadyMix format reduces setup time.

• Reduced risk of contamination from multiple pipet steps.

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL

Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable