The supplier recommends using a BCA protein assay when following the MAK340 procedure.
MAK340
Microsome Isolation Kit
sufficient for 50 isolation(s)
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About This Item
UNSPSC Code:
12161503
Pricing and availability is not currently available.
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usage
sufficient for 50 isolation(s)
storage temp.
−20°C
General description
Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily. Microsomal preparations are an affordable and convenient in vitro system for assessing Phase I biotransformation reactions, as they contain all of the xenobiotic-metabolizing CYP isozymes and the membrane-bound flavoenzymes (such as NADPH P450-Reductase and cytochrome b5) required for function of the multicomponent P450 enzyme system.
Suitability
Suitable for the isolation of Microsomes from mammalian glands and soft tissues such as liver, spleen, lungs etc. or cultured eukaryotic cell lines such as HepG2 human hepatic carcinoma cells
Principle
The Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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What is the preferred method for measuring protein for the MAK340 kit (Microsome Isolation assay) when preparing for Western Blot, as the bulletin recommends?
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