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M8948

Sigma-Aldrich

Monoclonal Anti-Myocardin antibody produced in mouse

~2.0 mg/mL, clone MCR54, purified immunoglobulin

Synonym(s):

Anti-MYCD, Anti-MYOCD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MCR54, monoclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2.0 mg/mL

technique(s)

western blot: 2.5-5 μg/mL using HEK-293T cells overexpressing myocardin

isotype

IgG2b

UniProt accession no.

Q8IZQ8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYOCD(93649)

General description

Monoclonal Anti-Myocardin (mouse IgG2b isotype) is derived from the hybridoma MCR54 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human myocardin. Myocardin (MYOCD) is a transcriptional coactivator.
Myocardin (MYOCD) is expressed in the muscles and cardiomyocytes. It is a co-transcriptional factor.

Application

Monoclonal Anti-Myocardin antibody produced in mouse has been used in western blot analysisimmunofluorescence.

Biochem/physiol Actions

Myocardin (MYOCD) takes part in the differentiation of vascular smooth muscle cells (VSMC). It is a co-factor of serum response factor (SRF) and regulates the activation of those genes which are dependent on SRF. MYOCD forms a complex with SRF on DNA.
Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor (SRF). It regulates the expression of a set of cardiac and smooth muscle-specific genes. Myocardin was implicated in the pathogenesis of atherosclerosis, Alzheimer′s disease, and malignant transformation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Myocardin in biology and disease
Miano JM
Journal of biomedical research, 29(1), 3-3 (2015)
Fibroblast growth factor 12 is a novel regulator of vascular smooth muscle cell plasticity and fate
Song SH, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 36(9), 1928-1936 (2016)
The serum response factor coactivator myocardin is required for vascular smooth muscle development
Li S, et al.
Proceedings of the National Academy of Sciences of the USA, 100(16), 9366-9370 (2003)
Pavneet Singh et al.
PloS one, 9(11), e112120-e112120 (2014-11-11)
De-differentiation of vascular smooth muscle cells (VSMCs) plays a critical role in the development of atherosclerosis, a chronic inflammatory disease involving various cytokines such as tumor necrosis factor-α (TNFα). Myocardin is a co-factor of serum response factor (SRF) and is
Amarnath Talasila et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(10), 2355-2365 (2013-07-05)
Myocardin, a potent transcriptional coactivator of serum response factor, is involved in vascular development and promotes a contractile smooth muscle phenotype. Myocardin levels are reduced during vascular injury, in association with phenotypic switching of smooth muscle cells (SMCs). However, the
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