This product is lyophilized from mouse ascites fluid. The total protein content is verified prior to lyophilization. The 15 mg/mL value is the pre-lyophilization total protein content. The Myeloma Protein-Densitometry is performed after lyophilization when reconstituted to 1ml in water. The 5 mg value is specific to the Myeloma Protein (IgG1 kappa) only.
M1398
IgG1, Kappa from murine myeloma
clone MOPC 31C, ascites fluid, lyophilized powder
Synonym(s):
Mouse IgG1-κ
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
clone
MOPC 31C, monoclonal
form
lyophilized powder
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
The MOPC 31C tumor line that produces mouse IgG1,k is a mineral oil-induced plasmacytoma originated and carried subcutaneously in BALB/c mice. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody.[1] An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense.[2] It is a glycoprotein. IgG is a major class of immunoglobulin.[3] Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.[3] Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.[4]
Application
IgG1, Kappa from murine myeloma has been used in immunohistochemistry.[5]
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. It provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[6]
Reconstitution
Reconstitute with 1 ml deionized water.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment
Westra I, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 233(9), 573-581 (1995)
The Laboratory Rat (1998)
Molecular Genetics of Immunoglobulin (1987)
The Immunoglobulins: Structure and Function (1998)
Flow-cytometric analysis of platelet-membrane glycoprotein expression and platelet activation.
Alison H Goodall et al.
Methods in molecular biology (Clifton, N.J.), 272, 225-253 (2004-07-01)
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Good morning, I have a question about product specification. Looks like 1vial contains about 5mg and the protein, correct? What does the 15mg/ml protein by Biuret info mean? Because if the reconstitution is done in 1mL, the highest conc. is 5mg/mL, no? Ty
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