Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

G0411

Sigma-Aldrich

Prionex® Highly purified Type A

aqueous solution

Synonym(s):

Gelatin, Prionex® Highly purified Type A

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9000-70-8
EC Number:
232-554-6
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.61
Pricing and availability is not currently available.

biological source

Porcine bone

Quality Level

200

sterility

aseptically processed

form

aqueous solution

technique(s)

ELISA: suitable
microbiological culture: suitable
western blot: suitable

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen.[1][2] The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.[3] Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue.[4] Gelatin is a biocompatible polymer.

Application

Prionex® Highly purified Type A has been used:
  • for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA[5]
  • as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry[6]
  • as a component of media for species differentiation in bacteriology[7]
  • as a delivery vehicle for the release of bioactive[8] molecules and in the generation of scaffolds for engineering applications[9]
  • in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo[10]

Legal Information

Prionex is a registered trademark of Pentapharm AG, Basel

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCW6198
MKCV5648
MKCW1833
MKCW6728
MKCV3801

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Photographic Processes and Materials
Encyclopedia of Physical Science and Technology, 91-132 (2001)
Food Gels
Encyclopedia of Materials: Science and Technology, 3231-3233 (2001)
Gels for Photographic Emulsions
Encyclopedia of Materials: Science and Technology, 3495-3497 (2001)
PCR Kit
PCR Primer: A Laboratory Manual (1995)
Amino Acid Composition of Gelatin from Ephinephelus sp.
Siregar GRM and Suprayitno E
IOSR Journal of Agriculture and veterinary Science, 12(4), 51-54 (2019)
View All Related Papers

Questions

1–2 of 2 Questions  

  1. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  2. What is the shelf life for G0411 (purified prionex) after opening? And is it okay to store 2-8C?

    1 answer

    1. After opening, this product may be stored at 2-8°C. The solution may require warming to room temperature for ease of use. With proper storage and aseptic handling, the stability will not be impacted after opening. Any change in color or turbidity may be an indication of contamination or degradation.

      This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link:
      https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service