The product is supplied in the form of a powder.
D1626
Deoxyribonucleic acid sodium salt from salmon testes
Synonym(s):
SS-DNA, smDNA, DNA
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Application
Deoxyribonucleic acid sodium salt has been used in a study to evaluate the cytotoxicity of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. Deoxyribonucleic acid sodium salt has also been used in a study to develop a simple method for extracting DNA from Cryptosporidium oocysts.
Deoxyribonucleic acid sodium salt from salmon testes has been used in basal medium for the anaerobic growth of several Bacillus mojavensis strains[1] It has been also used as a component of immunoprecipitation (IP) buffer for the Microplate-Based Matrix Chromatin Immunoprecipitation (ChIP).[2]
Deoxyribonucleic acid sodium salt from salmon testes has been used in basal medium for the anaerobic growth of several Bacillus mojavensis strains[1] It has been also used as a component of immunoprecipitation (IP) buffer for the Microplate-Based Matrix Chromatin Immunoprecipitation (ChIP).[2]
Salmon Sperm Deoxyribonucleic acid (SS-DNA) may be used to study physicochemical interactions of DNA with binding agents, intercalation agents, modification agents, detection agents and compaction agents. Salmon sperm DNA is used to study the loading capacity of magnetic mesoporous silica nanoparticles (M-MSNs) and binding capacities of polymer nanolayer architectures. SS-DNA may be used as substrate for development of DNA detection assays.
Linkage
Formerly listed as Type III
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Marie-Louise Ainalem et al.
Langmuir : the ACS journal of surfaces and colloids, 26(7), 4965-4976 (2009-12-05)
This study shows that DNA (linearized plasmid, 4331 base pairs and salmon sperm, 2000 base pairs, respectively) adsorbs to model membranes of zwitterionic liquid crystalline phospholipid bilayers in solutions containing divalent Ca(2+) cations, and also in solutions containing monovalent Na(+).
Sandra L McLellan et al.
Elementa (Washington, D.C.), 6 (2018-11-06)
Despite modern sewer system infrastructure, the release of sewage from deteriorating pipes and sewer overflows is a major water pollution problem in US cities, particularly in coastal watersheds that are highly developed with large human populations. We quantified fecal pollution
Thet Tun Aung et al.
The ocular surface, 15(4), 770-783 (2017-07-01)
The potential of slow-growing mycobacteria to form biofilms in human tissues contributes to the problem of establishing an effective treatment strategy. The purpose of this study was to examine new antibiotic strategies to enhance current treatment options for these infections.
Paul Cernak et al.
mBio, 9(5) (2018-09-27)
Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available
Natasha McStay et al.
Nucleic acids research, 45(2), 527-540 (2016-12-03)
Herein we report the synthesis of tripodal C
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Is the DNA solid (like a mesh) or is it a powder? The product number is D1626 Thank you.
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What us the molecular weight of product D1626?
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The molecular weight of this product has not been determined. This material is considered to be double-stranded. Historical data indicates that this material separates to two major bands during electrophoresis. One band indicating at least 9416 base pairs and one band at approximately 3350 base pairs. Note that each base pair has an estimated molecular weight of 650 daltons.
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This is a single-stranded or double-stranded?
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This product is a crude preparation. While it is not tested on a lot specific basis, it is considered to be double-stranded.
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What is the purity (%) of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
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This product is not tested for purity. Historically, the A260/A280 ratios have been >1.8. This value is generally consider to be an indication of purified DNA.
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What is the solubility of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
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Sigma tests solubility in water at 2 mg/mL. To prevent shearing of the large genomic DNA, this product should be dissolved in water (1 mg/mL) with no sonication or stirring. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solution stability of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
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Solutions of DNA have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.
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