storage temp.
−20°C
General description
This product consists of a primer set for the Arabidopsis housekeeping gene CBP20 (At5g44200). CBP20 encodes a nuclear cap-binding protein. It is intended for use as an internal control for Q-RT-PCR for expression analysis of any Arabidopsis gene.
Of our three control primer series for Arabidopsis gene expression, the CBP20 control primer is designed as a control for medium- to low-abundant gene expression. The other two control primers in the series are ubiquitin (UBC, Product Code C3240) also designed as a control for medium- to low-abundant gene expression and actin-2 (Product Code C3615) for high-abundant gene expression.
Of our three control primer series for Arabidopsis gene expression, the CBP20 control primer is designed as a control for medium- to low-abundant gene expression. The other two control primers in the series are ubiquitin (UBC, Product Code C3240) also designed as a control for medium- to low-abundant gene expression and actin-2 (Product Code C3615) for high-abundant gene expression.
Components
Each tube contains enough primer (forward or reverse) for 100 RT-PCR reactions (50 μl reaction volume)
- Forward Primer (Tm = 68 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2740.
- Reverse Primer (Tm = 66 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2865.
Suitability
Primer should be stored at -20 °C. Primers are stable at -20 °C for at least 1 year and can be frozen and thawed at least 10 times.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tatjana Trcek et al.
Molecular cell, 78(5), 941-950 (2020-05-29)
mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism
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