C1926
Monoclonal Anti-Collagen, Type IV antibody produced in mouse
clone COL-94, ascites fluid
Synonym(s):
Anti-Collagen IV, Collagen IV Detection Antibody, Mouse Anti-Collagen IV
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About This Item
Conjugate:
unconjugated
Clone:
COL-94, monoclonal
Application:
ARR, DB, ELISA (i), IHC (f), IHC (p)
Citations:
49
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
COL-94, monoclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, microarray: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL4A1(1282), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)
General description
Monoclonal Anti-Collagen Type IV (mouse IgG1 isotype) is derived from the COL-94 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen Type IV.
The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different α chain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain.
Immunogen
human collagen type IV
Application
Monoclonal Anti-Collagen, Type IV antibody produced in mouse has been used in
- indirect immunolabeling
- immunofluorescence staining
- western blotting
- immunohistochemistry
- immunocytochemistry
Biochem/physiol Actions
Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels.
Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eeva-Mari Jouhilahti et al.
The American journal of pathology, 178(2), 500-505 (2011-02-02)
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Karaer O, et al.
Fertility and Sterility, 83(6), 1797-1806 (2005)
Katelyn V Batterman et al.
Frontiers in immunology, 12, 607691-607691 (2021-03-06)
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Structure and Function of Basement Membranes
LeBleu VS et al
Exp. Biol. Med, 232, 1121-1129 (2007)
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Cui D, et al.
Toxicology Letters, 155(1), 73-85 (2005)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C1926-100UL | 04061837883484 |
| C1926-.2ML | 04061833469125 |
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