A0481

Anti-Axin1 (C-terminal) antibody produced in rabbit

1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1
• MDL number: MFCD02095870
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen ~130 kDa
• species reactivity: rat, mouse
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: 1.5 mg/mL
• technique(s): western blot: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1.
• UniProt accession no.: O35625
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AXIN1(8312) ; mouse ... Axin1(12005) ; rat ... Axin1(79257)

Description

General description

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC),,,.
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide

Immunogen

synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1.

Application

Anti-Axin1 (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1). The antibody has also been used for immunoblotting assays in astrocytoma cell lysates.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves