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45260

Sigma-Aldrich

Eosin B

for microscopy (Fl., Hist.), adsorption and fluorescent indicator

Synonym(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Linear Formula:
C20H6N2O9Br2Na2
CAS Number:
Molecular Weight:
624.06
Colour Index Number:
45400
Beilstein:
4121964
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

adsorption and fluorescent indicator
for microscopy (Fl., Hist.)

technique(s)

titration: suitable

λmax

509-524 nm

SMILES string

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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General description

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.

Application

Eosin B is an adsorption and fluorescent indicator in microscopy.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Xu Wang et al.
Electrophoresis, 31(23-24), 3808-3815 (2010-11-17)
In this study, we describe an effective visualizing technique for proteins in SDS-PAGE based on the organic dye, Eosin B, the sensitivity of which can be further strengthened by the addition of magnesium to the staining solution after electrophoresis. The
A A Waheed et al.
Analytical biochemistry, 287(1), 73-79 (2000-11-18)
Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than
Chloé E Atreya et al.
The Journal of biological chemistry, 278(16), 14092-14100 (2003-01-31)
Protozoal parasites are unusual in that their thymidylate synthase (TS) and dihydrofolate reductase (DHFR) enzymes exist on a single polypeptide. In an effort to probe the possibility of substrate channeling between the TS and DHFR active sites and to identify
Jishu Naskar et al.
Organic & biomolecular chemistry, 9(19), 6610-6615 (2011-08-13)
Self-assembling short peptides can offer an opportunity to make useful nano-/microstructures that find potential application in drug delivery. We report here the formation of multivesicular structures from self-assembling water-soluble synthetic amphiphilic dipeptides containing a glutamic acid residue at the C-terminus.
H J Shin et al.
Biochimica et biophysica acta, 1481(1), 139-146 (2000-09-27)
Among various dyes including congo red, thioflavin S, thioflavin T, eosin, rhodamine 6G, and phenol red, the eosin was the only dye that induced self-oligomerization of alpha-synuclein in the presence of a chemical coupling reagent of N-(ethoxycarbonyl)-2-ethoxy-1, 2-dihydroquinoline. To analyze

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