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3014

Sigma-Aldrich

Transcreener® GDP FI Assay

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About This Item

UNSPSC Code:
41122100
NACRES:
NA.81

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® GDP FI Assay is a competitive immunoassay for GDP with a red fluorescent intensity (FI) readout. Designed specifically for HTS and featuring a single addition mix-and-read format and room temperature reagent stability, the Transcreener® GDP FI Assay is compatible with plate readers typically found in research labs as well as those used in HTS core facilities. The 3014-A kit is formatted for 96-well plates while the 3014-1K and 3014- 10K kits are for 384-well and higher densities.

Able to accommodate GTP concentrations ranging from 0.1 to 1,000 μM, the Transcreener® GDP FI Assay provides excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less). Because the proprietary antibody used is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, including small G proteins. (Note that many commercially available GTPase preparations contain GDP as a stabilizer, which must be removed or diluted prior to use with this assay so as not to contribute to background signal.

View full Transcreener® product list

Quantity

3014-1K = 1,000 assay, 384-well
3014-10K = 10,000 assay, 384-well
3014-A = 200 assay, 96-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dong Won Kang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(24), 2002142-2002142 (2020-12-22)
NH3, essential for producing artificial fertilizers and several military and commercial products, is being produced at a large scale to satisfy increasing demands. The inevitable leakage of NH3 during its utilization, even in trace concentrations, poses significant environmental and health
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The aim of the present study was to investigate the protective effects and mechanisms of KLK1 on aging-related prostate alterations and search clues about the application of KLK1 to the treatment of human BPH. Thirty-six rats including 26 male wild-type
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Macrophages are highly phagocytic cells that utilize various pathogen recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). These PAMPs can be present within the microbe, such as bacterial CpG DNA, and are recognized by Toll-like receptor 9 (TLR9), a

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