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03038

Sigma-Aldrich

Tris(bathophenanthrolinedisulfonate)ruthenium(II) sodium salt solution

suitable for fluorescence, 20 mM in H2O

Synonym(s):

RuBPS, Rubin stain, Ruby stain, Ruthenium II tris (bathophenanthroline disulfonate), Ruthenium(II)-tris-(bathophenanthrolin-disulfonat) sodium salt solution

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About This Item

Empirical Formula (Hill Notation):
C72H42N6Na4O18RuS6
CAS Number:
301206-84-8
Molecular Weight:
1664.55
MDL number:
MFCD04040034
UNSPSC Code:
12171500
PubChem Substance ID:
329747544
NACRES:
NA.32

shelf life

limited shelf life, expiry date on the label

concentration

20 mM in H2O

solubility

H2O: soluble

fluorescence

λex 445 nm; λem 615 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[Na+].[Na+].[Na+].[Na+].[Ru++].[O-]S(=O)(=O)c1ccc(cc1)-c2ccnc3c2ccc4c(ccnc34)-c5ccc(cc5)S([O-])(=O)=O.[O-]S(=O)(=O)c6ccc(cc6)-c7ccnc8c7ccc9c(ccnc89)-c%10ccc(cc%10)S([O-])(=O)=O.[O-]S(=O)(=O)c%11ccc(cc%11)-c%12ccnc%13c%12ccc%14c(ccnc%13%14)-c%15ccc(cc%15)S([O-])(=O)=O

InChI

1S/3C24H16N2O6S2.4Na.Ru/c3*27-33(28,29)17-5-1-15(2-6-17)19-11-13-25-23-21(19)9-10-22-20(12-14-26-24(22)23)16-3-7-18(8-4-16)34(30,31)32;;;;;/h3*1-14H,(H,27,28,29)(H,30,31,32);;;;;/q;;;4*+1;+2/p-6

InChI key

AXMFYAOBYBXMLC-UHFFFAOYSA-H

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Application

Ruby Stain offers good sensitivity and minimal interference with MALDI-TOF analyis. It has been demonstrated as an effective fluorescent stain for the analysis of 2D-gel separated proteins .
Used for the detection of DNA

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
One package includes 50 μl concentrate. Dilution into 1 L water yields a 1 μM solution

Analysis Note

The dye can be excited with standard light sources. Excitation with 300 nm (UV light) or 473 nm (blue laser) is suitable.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBW3267
BCBV4462
BCBT5483
BCBP9926V
BCBM5616V

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