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A7284

Sigma-Aldrich

Bovine Serum Albumin solution

30%±2% in 0.85% sodium chloride, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

Quality Level

300

sterility

aseptically filled

Assay

28-32% protein basis (biuret)

form

solution

purified by

heat shock fractionation

contains

0.1% sodium azide as preservative

does not contain

stabilizer

origin

USA origin

concentration

28-32% protein (biuret)
30%±2% in 0.85% sodium chloride

technique(s)

ELISA: suitable

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is bound in a single chain cross-linked with 17 cysteine residues. It comprises three homologous, structurally different domains and two sub-domains each. This globular, α-helical, non-glycosylated protein is produced in the liver.

Application

Bovine Serum Albumin solution has been used:
  • to study the effects of plasma protein on sulfated glycosaminoglycans (sGAGs)
  • to histologically evaluate the implanted allogeneic mesenchymal stem cells (MSCs) alginate constructs
  • as a component of phosphate-buffered saline (PBS) to treat red blood cells (RBC) for sample preparation

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most widely used blocking agent in enzyme-linked immunosorbent assay (ELISA). It is also the main constituent of cell culture media. BSA promotes the differentiation of human embryonic stem cells (hESC) differentiation and assists the circulation of drugs, hormones, and fatty acids.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000395520
0000351249
0000351251
0000385521
0000373449

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R Zhao et al.
Biorheology, 47(3-4), 193-203 (2010-11-19)
The accumulation of platelets near the blood vessel wall or artificial surface is an important factor in the cascade of events responsible for coagulation and/or thrombosis. In small blood vessels and flow channels this phenomenon has been attributed to the
M J Leijs et al.
European cells & materials, 33, 43-58 (2017-02-01)
Bone marrow derived mesenchymal stem cells (MSCs) have immunomodulatory and trophic capacities. For therapeutic application in local chronic inflammatory diseases, MSCs, preferably of allogeneic origin, have to retain immunomodulatory properties. This might be achieved by encapsulation of MSCs in a
Emily M Thrash et al.
STAR protocols, 1(2), 100055-100055 (2020-10-29)
As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow for high-throughput staining that generates reproducible data for up
Derry C Roopenian et al.
mAbs, 7(2), 344-351 (2015-02-06)
Serum albumin is the major determinant of blood colloidal osmotic pressure acting as a depot and distributor of compounds including drugs. In humans, serum albumin exhibits an unusually long half-life mainly due to protection from catabolism by neonatal Fc receptor
Brent L Wood
Current protocols in cytometry, 93(1), e73-e73 (2020-04-21)
The identification of residual leukemia following therapy, termed minimal or measurable residual disease (MRD), has emerged as one of the most important prognostic factors for patients with acute leukemia, including acute myeloid leukemia (AML). Flow cytometry is a preferred method
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