49971
D-Lactic Dehydrogenase from Lactobacillus leichmannii
suspension, yellow, ~1000 U/mL
Synonym(s):
(R)-Lactate:NAD+ oxidoreductase, D-Lactate Dehydrogenase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
biological source
bacterial (Lactobacillus leichmannii)
form
suspension
specific activity
≥30 U/mg protein
concentration
~1000 U/mL
impurities
L-Lactat Dehydrogenase, none detected
3.2 M Ammonium sulfate solution (pH ~6.2)
color
yellow
storage temp.
2-8°C
Application
In the food industry, the primary catalysis is coupled to conversion of NADH and H+ to NAD+ with diaphorase coupled with converting the non-fluorescent resazurin to the highly fluorescent substance resorufin to measure the content of D-lactate in food products.
Biochem/physiol Actions
D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD+. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD+ to NADH.
D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD+. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD+ to NADH.
Unit Definition
1 U corresponds to the amount of enzyme which will reduce 1 μmol of pyruvate to D-lactate per minute at pH 7.0 and 25 °C
Physical form
only partially soluble in water or buffer
replaced by
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Martin Engqvist et al.
The Journal of biological chemistry, 284(37), 25026-25037 (2009-07-10)
The Arabidopsis thaliana locus At5g06580 encodes an ortholog to Saccharomyces cerevisiae d-lactate dehydrogenase (AtD-LDH). The recombinant protein is a homodimer of 59-kDa subunits with one FAD per monomer. A substrate screen indicated that AtD-LDH catalyzes the oxidation of d- and
Akos T Kovács et al.
Applied and environmental microbiology, 76(12), 4085-4088 (2010-04-20)
Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay
Fei Su et al.
Journal of bacteriology, 193(17), 4563-4564 (2011-06-28)
Bacillus coagulans 2-6 is an efficient producer of lactic acid. The genome of B. coagulans 2-6 has the smallest genome among the members of the genus Bacillus known to date. The frameshift mutation at the start of the d-lactate dehydrogenase
Takenori Shibahara et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 67(Pt 11), 1425-1427 (2011-11-22)
A dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.4
R Lamichhane-Khadka et al.
Journal of applied microbiology, 105(6), 1973-1981 (2009-01-06)
To identify factors associated with the Staphylococcus aureus pine-oil disinfectant-reduced-susceptibility (PD(RS)) mechanism and to describe one possible PD(RS) model. Comparative genomic sequencing (CGS) and microarray analysis were utilized to detect mutations and transcriptome alterations that occur in a S. aureus
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