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30433

Sigma-Aldrich

DAPER DNA precipitation reagent

for fluorescence, ≥95.0% (HPCE)

Synonym(s):

N,N′-Bis[3-(dimethylamino)propyl]perylene-3,4,9,10-tetracarboxylic diimide

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About This Item

Empirical Formula (Hill Notation):
C34H32N4O4
CAS Number:
Molecular Weight:
560.64
Beilstein:
9243299
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:

grade

for fluorescence

Assay

≥95.0% (HPCE)

fluorescence

λex 520 nm; λem 540 nm in ethanol

SMILES string

CN(C)CCCN1C(=O)c2ccc3c4ccc5C(=O)N(CCCN(C)C)C(=O)c6ccc(c7ccc(C1=O)c2c37)c4c56

Physical form

1 mM solution in 10 mM 2-(N-morpholino)ethanesulfonic acid pH 6.5

Analysis Note

In addition to the emission maximum at 540 nm, there are lower maxima at 576 nm and 630 nm.

Other Notes

Rapid precipitation and quantitation of trace amounts of DNA

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Z R Liu et al.
Analytical biochemistry, 236(1), 139-145 (1996-04-05)
A novel dicationic dye with a polycyclic aromatic perylene core and flexible cationic side chains- N,N'-bis[3, 3'-(dimethylamino)propylamine]-3,4,9,10-perylenetetracarboxylic diimide-termed "DAPER," was synthesized and characterized. The dye appears to exist in a highly stacked form in aqueous solution. DAPER precipitates extremely low

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