30433
DAPER DNA precipitation reagent
for fluorescence, ≥95.0% (HPCE)
Synonym(s):
N,N′-Bis[3-(dimethylamino)propyl]perylene-3,4,9,10-tetracarboxylic diimide
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About This Item
grade
for fluorescence
Assay
≥95.0% (HPCE)
fluorescence
λex 520 nm; λem 540 nm in ethanol
SMILES string
CN(C)CCCN1C(=O)c2ccc3c4ccc5C(=O)N(CCCN(C)C)C(=O)c6ccc(c7ccc(C1=O)c2c37)c4c56
Physical form
1 mM solution in 10 mM 2-(N-morpholino)ethanesulfonic acid pH 6.5
Analysis Note
In addition to the emission maximum at 540 nm, there are lower maxima at 576 nm and 630 nm.
Other Notes
Rapid precipitation and quantitation of trace amounts of DNA
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Analytical biochemistry, 236(1), 139-145 (1996-04-05)
A novel dicationic dye with a polycyclic aromatic perylene core and flexible cationic side chains- N,N'-bis[3, 3'-(dimethylamino)propylamine]-3,4,9,10-perylenetetracarboxylic diimide-termed "DAPER," was synthesized and characterized. The dye appears to exist in a highly stacked form in aqueous solution. DAPER precipitates extremely low
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