Skip to Content
Merck
All Photos(1)

Documents

06432344001

Roche

DNA Fragmentation Imaging Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

General description

The DNA Fragmentation Imaging Kit is a simple and rapid TUNEL assay for detecting apoptosis induction in mammalian cells. DNA fragmentation, detected using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP, is measured by fluorescence microscopy or an automated imaging platform, such as the Cellavista System. The kit uses a dye to stain the nuclei of living and dead cells for quantifying total and apoptotic cell numbers, and calculating the percentage of apoptotic cells.

Application

Programmed cell death (apoptosis) is a key pathway in mammalian cells during tissue homeostasis. Cell death malfunction has been implicated in pathology. Quantification of caspase-triggered DNA fragmentation is one of the principal techniques used to detect apoptosis induction. The DNA Fragmentation Imaging Kit performs the TUNEL assay for accurate and fast quantitative fluorescence detection using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP. The kit is ideal for measuring apoptosis in medium to high throughput cellular workflows.
  • Quickly determine apoptosis induction for cell-based workflows in life science research.
  • Reliably perform screening assays in product development, e.g., cancer research, pre-clinical safety.
  • Accurately measure malignant cell sensitivity in cancer research.

Features and Benefits

  • Optimized for data evaluation with Roche′s Cellavista System. Obtain reproducible results using automated imaging with this system.
  • Saves time and resources: Total assay time, including analysis, for a 96-well microplate is 85 minutes.
  • Reduced risk of losing cells with fewer washing steps in an improved protocol.

Packaging

1 kit containing 3 components

Quality

The kit is function tested using a cellular model (HeLa cells treated with antimycin A).

Principle

Labeling of apoptotic cells
Genomic DNA cleavage during apoptosis produces double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), as well as single strand breaks (nicks) in high molecular weight DNA. These DNA strand breaks are identified by labeling free 3′-OH termini with modified nucleotides in an enzymatic assay known as the TUNEL reaction. The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. TUNEL assays discriminate apoptosis from necrosis, and from primary DNA strand breaks induced by cytostatic drugs or irradiation. The DNA Fragmentation Imaging Kit labels DNA strand breaks using terminal deoxynucleotidyl transferase (TdT). TdT catalyzes polymerization of labeled nucleotides to free 3′-OH DNA ends in a template-independent manner using the TUNEL reaction. Fluorescein incorporated in nucleotide polymers is detected and quantified using fluorescence microscopy.
Staining of nuclei
Hoechst 33342 is a cell-permeable stain that binds DNA. It is used to stain nuclei of living or dead cells.

Preparation Note

Storage conditions (working solution): The Reaction Solution created by mixing the Enzyme Solution and Label Solution (Vial 2 and Vial 3), in Step 6 of the experiment protocol, cannot be stored. The Reaction Solution should be prepared just before use. Discard remaining unused Reaction Solution.

Storage and Stability

Store at 15 to 25 °C. (Protect from exposure to light. Store dry in dark.)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Nuclei Dye (blue cap), for fluorimetric determination of cell count based on labeling of cell nuclei

  • Enzyme Solution (yellow cap), for labelling of free 3′-OH termini of DNA fragements with fluorescein dUTP

  • Label Solution (clear cap), containing a nucleotid mix with fluorescein dUTP in reaction buffer

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B - Muta. 2 Inhalation

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Hao Li et al.
Asian journal of andrology, 20(4), 342-348 (2018-01-11)
Calpain activation contributes to hyperglycemia-induced endothelial dysfunction and apoptosis. This study was designed to investigate the role of calpain inhibition in improving diabetic erectile dysfunction (ED) in mice. Thirty-eight-week-old male C57BL/6J mice were divided into three groups: (1) nondiabetic control
Alfonso Rubio-Navarro et al.
Frontiers in pharmacology, 10, 740-740 (2019-07-25)
Massive intravascular hemolysis is associated with acute kidney injury (AKI). Nuclear factor erythroid-2-related factor 2 (Nrf2) plays a central role in the defense against oxidative stress by activating the expression of antioxidant proteins. We investigated the role of Nrf2 in
Rui Li et al.
PeerJ, 7, e7192-e7192 (2019-07-19)
The growth and function of seminal vesicle are dependent on androgen. This study was conducted to investigate the role of oxidative stress in castration-induced seminal vesicle atrophy and to explore the effects of curcumin, an antioxidant extracted from rhizome of
Pierpaolo Moscariello et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 5(5), 1700897-1700897 (2018-06-08)
Neurological disorders are undoubtedly among the most alarming diseases humans might face. In treatment of neurological disorders, the blood-brain barrier (BBB) is a challenging obstacle preventing drug penetration into the brain. Advances in dendrimer chemistry for central nervous system (CNS)
Iris Chiara Salaroglio et al.
Journal of experimental & clinical cancer research : CR, 41(1), 75-75 (2022-02-25)
The combination of pemetrexed and cisplatin remains the reference first-line systemic therapy for malignant pleural mesothelioma (MPM). Its activity is moderate because of tumor aggressiveness, immune-suppressive environment and resistance to chemotherapy-induced immunogenic cell death (ICD). Preliminary and limited findings suggest

Articles

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service