Here are the troubleshooting steps for the dialysis set-up and nitrogen escape from the top lead seal:
1) Before starting the diafiltration, customers should run gas pressure directly to the stirred cell for 5-10 seconds with the selector valve in “gas” mode.
2) Petroleum jelly will likely be required to completely seal the top gasket of the stirred cell.
UFSC05001
Amicon® Stirred Cell 50mL
This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit.
Synonym(s):
Stirred Cell
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About This Item
UNSPSC Code:
41104903
eCl@ss:
32031690
Pricing and availability is not currently available.
Recommended Products
material
316 stainless steel ring(s) (stir bar retaining)
316 stainless steel washer
Nylon connections (Luer on filtrate tubing)
Tygon® tubing (filtrate; E-3603)
polyacetal base
polyacetal support (for stir bar)
polyethylene tubing (pressure)
polysulfone body
polysulfone cap
polysulfone holder (for membrane)
polysulfone stir bar(s)
silicone O-ring
silicone gasket
Quality Level
product line
Amicon®
manufacturer/tradename
Amicon®
parameter
50 mL process volume
technique(s)
protein clean-up: suitable (desalting)
protein purification: suitable (concentration)
H
11.7 cm (4.6 in.)
filter diam.
44.5 mm
filtration area
13.4 cm2
shipped in
ambient
Application
This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit.
Principle
Pressure
Linkage
Replaces: 5122
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
Tygon is a registered trademark of Saint-Gobain Performance Plastics Corp.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
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How can I troubleshoot the issue with ufsc05001 where, during the process of diafiltration to push liquid into the stirred cell, the liquid is added too fast and overflows?
1 answer-
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Is the membrane holder for the Amicon Stirred Cell UFSC05001 sold as a replacement part?
1 answer-
No, the membrane holder component of the UFSC Amicon Stirred Cells is not sold separately.
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