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ST1027

Sigma-Aldrich

Anti-Acetylated Lysine Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys)

General description

Anti-Acetylated Lysine, rabbit polyclonal antibody that recognizes acetylated lysine in UV-treated HEK293 cells. It is validated for ELISA, Western blotting, immunoprecipitation, & paraffin sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes acetylated lysine-containing proteins.
Recognizes acetylated lysine residues in UV-treated HEK293 cells.

Immunogen

synthetic acetylated-lysine

Application


ELISA (1:1000)
Immunoblotting (1:1000)
Immunoprecipitation (1:100)
Paraffin Sections (1:50)

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
HEK293 cells treated with UV

Other Notes

Yu, X., et al. 2002. J. Natl. Cancer Inst.94, 504.
Mal, A., et al. 2001. EMBO J.20, 1739.
Sano, Y. and Ishii, S. 2001. J. Biol. Chem.276, 3674.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chao Yang et al.
The Journal of biological chemistry, 287(42), 34917-34926 (2012-08-25)
The MYST family of histone acetyltransferases (HATs) plays critical roles in diverse cellular processes, such as the epigenetic regulation of gene expression. Lysine autoacetylation of the MYST HATs has recently received considerable attention. Nonetheless, the mechanism and function of the
Ling Cai et al.
Cell reports, 4(6), 1063-1071 (2013-09-17)
Ribosome biogenesis requires an enormous commitment of energy and resources in growing cells. In budding yeast, the transcriptional coactivator Ifh1p is an essential regulator of ribosomal protein (RP) gene transcription. Here, we report that Ifh1p is dynamically acetylated and phosphorylated
Jeong-Hoon Kim et al.
Genes & development, 24(24), 2766-2771 (2010-12-17)
The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of
Jingjing Zhang et al.
EMBO reports, 18(8), 1294-1305 (2017-06-16)
Two sister chromatids must be held together by a cohesion process from their synthesis during S phase to segregation in anaphase. Despite its pivotal role in accurate chromosome segregation, how cohesion is established remains elusive. Here, we demonstrate that yeast
Soumya Rudra et al.
PloS one, 8(9), e75435-e75435 (2013-10-03)
The conserved family of cohesin proteins that mediate sister chromatid cohesion requires Scc2, Scc4 for chromatin-association and Eco1/Ctf7 for conversion to a tethering competent state. A popular model, based on the notion that cohesins form huge ring-like structures, is that
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