Vacuum filter cups are commonly used in the industry to degas solutions. For some liquid chromatography systems, applying a vacuum while filtering the mobile phase may remove enough gas to prevent issues.
S2GPT02RE
Millipore® Steritop® Vacuum Bottle Top Filter
pore size 0.22 μm, polyethersulfone membrane, funnel capacity 250 mL, pack of 12 ea
Synonym(s):
SCGPT02RE, Steritop Threaded Bottle Top Filter, bottle top sterile filter unit, bottle top vacuum filter, bottle top vacuum filtering unit
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About This Item
UNSPSC Code:
41104914
eCl@ss:
32031623
NACRES:
NB.24
Pricing and availability is not currently available.
Recommended Products
material
polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Steritop®
feature
hydrophilic
packaging
pack of 12 ea
parameter
45 °C max. temp.
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
95 mm
capacity
250 mL
diam.
12.5 cm
filter diam.
73 mm
filtration area
40 cm2
funnel capacity
250 mL
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet funnel
45 mm outlet thread(s)
shipped in
ambient
General description
This item is a direct replacement for SCGPT02RE
Work with ease and filter with confidence with Stericup® and Steritop® Filtration Systems.
Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop® 250 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop® 250 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Application
For Research Use Only
Stericup® and Steritop® systems are designed to reduce foaming and protein denaturation while maximizing flow and can be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
Legal Information
EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
STERITOP is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Related Content
This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
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Can you use filtration units to filter and also degas solutions?
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
1 answer-
You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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What Bacterial Endotoxins Test is used?
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The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What is the maximum vacuum pressure for filtration?
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700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
1 answer-
All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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Are Stericup® and Steritop® filters GMP-certified?
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No, Stericup® and Steritop® devices are not GMP-approved.
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Can Stericup® and Steritop® filters be reused?
1 answer-
All Stericup® and Steritop® filters were developed for single use, disposable processing.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
1 answer-
No, all Stericup® and Steritop® devices are Research Use Only.
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What is the expiration date of Stericup® and Steritop® filters?
1 answer-
Typically 3 years from sterilization.
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