MABF466

Anti-KLRG1 Antibody (mouse), PE, clone 2F1

clone 2F1, 0.2 mg/mL, from hamster(Syrian)

• Synonym(s): Killer cell lectin-like receptor subfamily G member 1, Mast cell function-associated antigen 2F1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: hamster (Syrian)
• Quality Level: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2F1, monoclonal
• species reactivity: mouse
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG
• UniProt accession no.: O88713
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Klrg1(50928)

Description

General description

The 2F1 antibody reacts with mouse KLRG1 (Killer cell Lectin-like Receptor G1). This 30-38 kDa homodimeric receptor may be expressed by activated, mature NK cells and by effector/memory T cells, with potentially different roles in each cell type. KLRG1 can regulate, in an inhibitory fashion, the development and effector functions of NK cells, and is often cited as a senescence or terminal differentiation marker for T cells. Ligands for KLRG1 include members of the cadherin family of adhesion molecules, specifically N-Cadherin, E-Cadherin, and R-Cadherin. These interactions may induce bidirectional, immunosuppressive signaling in both KLRG- and Cadherin-expressing cells. A more recently identified role for KLRG1-Cadherin signaling in tissue organization, e.g. in cardiac angiogenesis, expands the function of these interactions beyond immunosuppression of immune cells. (Bouchentouf et al. 2010. J. Immunol. 185: 7014-7025).The 2F1 antibody may be used as a phenotypic marker for KLRG1 in mouse, frequently in combination with Anti-Mouse CD127 antibody (clone A7R34), for identification of effector T cell populations.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This golden syrian hamster monoclonal antibody Anti-KLRG1 (mouse), PE, clone 2F1 validated for use in IP & Flow Cytometry for the detection of KLRG1.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected KLRG1 in one million C57Bl/6 splenocytes.

Physical form

Purified Golden Syrian Hamster Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg