MABF150D

Anti-IL-2 (mouse), clone JES6-5H4, biotin conjugate Antibody

clone JES6-5H4, from rat

• Synonym(s): Interleukin-2 (IL-2), T-cell growth factor (TCGF), IL-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: JES6-5H4, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• UniProt accession no.: P04351
• target post-translational modification: unmodified
• Gene Information: mouse ... Il2(16183)

Description

General description

The JES6-5H4 antibody binds to mouse Interleukin-2 (IL-2), a 17 kDa cytokine that is secreted by activated T cells. This cytokine acts as an autocrine stimulatory factor for T cell proliferation, differentiation and survival, as well as being an effective activator of B cells and inducer of NK cell cytotoxic functions. IL-2 plays a key modulatory role in the differentiation of T cells toward either Th17 or T regulatory (Treg) cell types and is important for Treg cell function. The IL-2 receptor (IL-2R) is a complex consisting of an IL-2R alpha chain (CD25), along with the IL-2R beta chain (CD122) and the common gamma chain (CD132). IL-2 may also bind with low affinity to the T cell surface protein CD25 alone, although this interaction does not induce cell signaling.The JES6-5H4 antibody is extensively used for intracellular detection of IL-2 by flow cytometry, for detection of soluble cytokine in ELISA, and as a neutralizing reagent in vitro and in vivo.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rat monoclonal Anti-IL-2 Antibody (mouse), clone JES6-5H4, biotin conjugate, Cat. No. MABF1508 is validated for use in Flow Cytometry, for the detection of IL-2.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected IL-2 in one million C57Bl/6 splenocytes.

Physical form

Format: Purified

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg