MAB390

Anti-p75 LNGFR Antibody, Saporin conjugated, clone 192

clone 192, Chemicon^®, from mouse

• Synonym(s): CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: saporin
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 192, monoclonal
• species reactivity: rat
• should not react with: human, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin)
• isotype: IgG1
• NCBI accession no.: NM_002507.2
• UniProt accession no.: P08138
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NGFR(4804)

Description

General description

The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate. The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4. Nerve growth factor, the prototypical growth factor, is a protein secreted by a neuron′s target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.

Specificity

Recognizes the rat NGF receptor (p75, low affinity Neurotrophin Receptor). MAB365 is the monoclonal antibody used for MAB390-SAP.

Immunogen

p75-expressing cells

Application

Immunohistochemistry (Paraffin):
A previous lot of this antibody was used in IH(P).

Immunocytochemistry:
A previous lot of this antibody was used in IC.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Neurochemistry & Neurotrophins

This Anti-p75 Low Affinity Nerve Growth Factor Receptor Antibody, Saporin conjugated, clone 192 is validated for use in ICC, IHC(P), FUNC for the detection of p75 Low Affinity Nerve Growth Factor Receptor.

Physical form

Protein A purified

Saporin Conjugated. 192-IgG-SAP is sterile filtered and presented in Dulbecco′s PBS with no preservative.

Storage and Stability

Centrifuge material at low speed in microfuge to ensure all of the solution is at the bottom of the tube. Vortex gently. Long term storage at -70ºC or below. Short term storage at -20ºC or 2-8ºC with appropriate antibacterial agent if compatible with the intended application. The sterile conjugate is stable at 2-8ºC for several months. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability if compatible with the intended application. This immunotoxin may be handled safely using standard laboratory procedures as the cross-species reactivity between these anti-rat p75 antibodies and the human form of p75 is negligible.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable