MAB3404-C
Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)
clone CK2, 1 mg/mL, from mouse
Synonym(s):
Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CK2, monoclonal
species reactivity
human
concentration
1 mg/mL
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRT18(3875)
General description
Cytokeratin 18, also known as Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, CK-18, Keratin-18, or K18, and encoded by the gene KRT18/CYK18/PIG46, is involved in the uptake of thrombin-anti-thrombin complexes by hepatic cells. When the protein is phosphorylated it plays a role in filament reorganization. Cytokeratin-18 together keratin-8 and IL-6 are involved in skin barrier protection too, and cytokeratin-18 is often complexed with Cytokeratin 8. Cytokeratin-18 is expressed in colon, placenta, liver epithelia and many other tissues. Cytokeratin 18 in epithelial cells is cleaved by caspases during apoptosis to produce apoptotic specific CK-18 fragment that is the basis of many apoptosis detection assays.
Specificity
The antibody reacts with human cytokeratin 18 and is used for staining research samples of a wide variety of simple epithelia and simple glandular epithelia (e. g. intestinal, respiratory and urinary epithelia) and transitional epithelia (e. g. bladder), but stratified squamous epithelia are not stained (e. g. esophagus, epidermis).
Immunogen
Cytoskeletal preparation of HeLa cells expressing human Cytokeratin 18.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cytokeratin 18 in human placenta tissue.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
This Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.
Quality
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cytokeratin 18 in human colorectal cancer tissue.
Target description
~48 kDa calculated
Linkage
Replaces: 04-586
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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