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MAB16988

Sigma-Aldrich

Anti-Hepatitis B Virus Antibody, core Antigen, clone C1-5, a.a. 74-89

ascites fluid, clone C1-5, Chemicon®

Synonym(s):

HBcAg

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

300

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C1-5, monoclonal

species reactivity

human, avian

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

shipped in

wet ice

Specificity

Reacts with HBcAg (epitope recognized - around amino acid positions 70-80, major C determinant).

SPECIES REACTIVITIES:

Cross-reacts with woodchuck hepatitis virus core antigen (WHcAg), but not with duck hepatitis B virus core antigen (DHcAg).

Immunogen

Epitope: Core Antigen
Recombinant purified HBcAg from E.coli

Application

Anti-Hepatitis B Virus Antibody, core antigen, clone C1-5, a.a. 74-89 is an antibody against Hepatitis B Virus for use in ELISA, IF, IP, WB, IC, IH.
ELISA, immunoblotting, immunoprecipitation, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells. Optimal working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Physical form

Ascitic fluid. Liquid in DMEM, containing 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Assembly Pathway of Hepatitis B Core Virus-like Particles from Genetically Fused Dimers.
Holmes, K; Shepherd, DA; Ashcroft, AE; Whelan, M; Rowlands, DJ; Stonehouse, NJ
The Journal of Biological Chemistry null
Cihan Makbul et al.
Microorganisms, 9(5) (2021-05-06)
Hepatitis B virus is a major human pathogen, which forms enveloped virus particles. During viral maturation, membrane-bound hepatitis B surface proteins package hepatitis B core protein capsids. This process is intercepted by certain peptides with an "LLGRMKG" motif that binds
A heteroaryldihydropyrimidine activates and can misdirect hepatitis B virus capsid assembly
Stray, Stephen J, et al
Proceedings of the National Academy of Sciences of the USA, 102, 8138-8143 (2005)
The structure of the variable regions of mouse monoclonal antibodies to hepatitis B virus core antigen.
Skrivelis, V, et al.
Scandinavian Journal of Immunology, 37, 637-643 (1993)
Differentiation of core gene products of the hepatitis B virus in infected liver tissue using monoclonal antibodies
Naoumov, N V, et al
Journal of Medical Virology, 53, 127-138 (1997)
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