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ABN2191

Sigma-Aldrich

Anti-Calretinin

from rabbit, purified by affinity chromatography

Synonym(s):

CR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CALB2(794)

Related Categories

General description

Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has multiple calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7 20 µm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070).

Specificity

This rabbit polyclonal antibody detects Calretinin in human and rat cells. It targets an epitope within 99 amino acids from the internal region.

Immunogen

Epitope: domain of:
GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin.

Application

Anti-Calretinin Antibody, Cat. No. ABN2191, is a highly specific rabbit polyclonal antibody that targets Calretinin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Calretinin in rat brain tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calretinin in human cerebral cortex and rat cerebellum tissue sections.

Quality

Evaluated by Western Blotting in human fetal brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Calretinin in human fetal brain tissue lysate.

Target description

~31 kDa observed; 31.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jennifer C Miguel et al.
Frontiers in aging neuroscience, 13, 645334-645334 (2021-03-30)
Cerebellar hypoplasia is a major characteristic of the Down syndrome (DS) brain. However, the consequences of trisomy upon cerebellar Purkinje cells (PC) and interneurons in DS are unclear. The present study performed a quantitative and qualitative analysis of cerebellar neurons

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