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AB2963

Sigma-Aldrich

Anti-MMP-3 Antibody, NT

from rabbit, purified by affinity chromatography

Synonym(s):

matrix metallopeptidase 3 (stromelysin 1, progelatinase), matrix metalloproteinase 3 (stromelysin 1, progelatinase), Matrix metalloproteinase-3, transin-1, proteoglycanase, stromelysin-1, Transin-13

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rhesus macaque, human

species reactivity (predicted by homology)

chimpanzee (100% sequence homology), rhesus monkey (100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_002413

UniProt accession no.

P08254

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MMP3(4314)

General description

MMP-3 (matrix metalloproteinase-3), also known as stromelysin-1, is a member of the matrix metalloproteinase (MMP) family of zinc endopeptidases. MMP’s are required for removal of extracellular matrix machinery during embryo development, morphogenesis and tissue remodeling. Endogenous tissue inhibitors of metalloproteinases (TIMPs) are responsible for regulating MMP-3. Any disruption of this system can cause diseases such as arthritis, metastasis, atherosclerosis and tumor growth.

Specificity

Other homologies: Mouse and rat (64% sequence homology).
This antibody recognizes MMP-3 at the N-terminus.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human N-terminal MMP-3.

Application

Detect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected MMP-3 in colorectal carcinoma and ductal carcinoma tissues.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs

Quality

Evaluated by Western Blot in PMA-treated HL-60 concentrated media.

Western Blot Analysis: 0.1-1 µg/mL of this antibody detects MMP-3 in 10 µg of PMA-treated HL-60 concentrated media.

Target description

~58 kDa observed.
An uncharacterized band may be seen at ~78 kDa

Linkage

Replaces: AB812

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
PMA-treated HL-60 concentrated media

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A V Stone et al.
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Meniscus injury increases osteoarthritis risk but its pathobiology in osteoarthritis is unclear. We hypothesized that older adult vervet monkeys would exhibit knee osteoarthritic changes and the degenerative menisci from these animals would secrete matrix metalloproteinases (MMPs) and pro-inflammatory cytokines that
A V Stone et al.
Osteoarthritis and cartilage, 22(2), 264-274 (2013-12-10)
Meniscus injury increases the risk of osteoarthritis; however, the biologic mechanism remains unknown. We hypothesized that pro-inflammatory stimulation of meniscus would increase production of matrix-degrading enzymes, cytokines and chemokines which cause joint tissue destruction and could contribute to osteoarthritis development.
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