565764

γ-Secretase Substrate, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

• Synonym(s): γ-Secretase Substrate, Fluorogenic, NMA-GGVVIATVK(DNP)- DR DR DR-NH₂
• Empirical Formula (Hill Notation): C₇₀H₁₁₆N₂₆O₁₈
• Molecular Weight: 1609.83
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL; 5% acetic acid: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence.

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.

Biochem/physiol Actions

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂

Reconstitution

Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable