444234

MMP-9-Lipocalin-TIMP-1 Complex, Human Neutrophil

• Synonym(s): Matrix Metalloproteinase 9-Lipocalin-TIMP-1 Complex
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥600 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• foreign activity: other MMP activity, none detected
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the Pro-MMP-9/TIMP-1 complex, acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase, with reduced activity.

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the progelatinase B/TIMP-1 complex in acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase with reduced activity.

Note: 1 mU = 1 milliunit.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable