17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
Synonym(s):
Catch and release system, Immunoprecipitation kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NB.22
Recommended Products
species reactivity (predicted by homology)
all
Quality Level
manufacturer/tradename
Catch And Release
Upstate®
technique(s)
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
General description
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Application
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Packaging
50 immunoprecipitations
Components
Catch and Release Wash Buffer, 10X (Cat.# 20-210)
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Quality
routinely evaluated by immunoprecipitation.
Storage and Stability
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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