1.04119

Osmium(VIII) oxide

for microscopy

• Synonym(s): Osmium(VIII) oxide
• Empirical Formula (Hill Notation): O₄Os
• CAS Number: 20816-12-0
• Molecular Weight: 254.23
• MDL number: MFCD00011150
• UNSPSC Code: 12352303

Properties

• vapor pressure: 10 hPa ( 20 °C)
• Quality Level: 200
• potency: 15 mg/kg LD₅₀, oral (Rat)
• color: yellow
• pH: 6 (20 °C, 25 g/L in H₂O)
• bp: 130 °C/1013 hPa
• mp: 40 °C (decomposition)
• solubility: 65 g/L
• density: 4.91 g/cm³ at 20 °C
• bulk density: 1500 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 15-25°C
• SMILES string: [Os](=O)(=O)(=O)=O
• InChI: 1S/4O.Os
• InChI key: VUVGYHUDAICLFK-UHFFFAOYSA-N

Description

General description

Osmium tetroxide for microscopy can be used to visualize unsaturated lipids. A part of the lipids is immediately blackened by the influence of osmic acid solution. The immediate black coloration results from the -C=C- double bond of the unsaturated fatty acid. The fatty acids that are made brownish at first can be blackened by treatment with 60-70% alcohol. For electron microscopy or semithin sectioning the glutardialdehyde-fixed specimens are post-fixed with osmium(VIII) oxide. Lipid inclusions appear black on a yellow to brown background.

Application

for microscopy

Analysis Note

Identity (Raman-spectrum): conforms

Safety Information

• Pictograms: GHS05,GHS06
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330 - H314
• Precautionary Statements: P260 - P271 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable