07-1517
Anti-phospho-IRS-2 (Ser388) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Insulin receptor substrate 2, IRS-2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer388)
Gene Information
human ... IRS2(8660)
General description
Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
Specificity
This antibody recognizes IRS-2 phosphorylated at Ser388.
Immunogen
Epitope: Phosphorylated Ser388
KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.
Application
Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.
Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Quality
Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Target description
~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and insulin treated HEK293 cell lysates
Untreated and insulin treated HEK293 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rania Alaaeldin et al.
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Insulin resistance contributes to several disorders including type 2 diabetes and cardiovascular diseases. Carpachromene is a natural active compound that inhibits α-glucosidase enzyme. The aim of the present study is to investigate the potential activity of carpachromene on glucose consumption
K I Rother et al.
The Journal of biological chemistry, 273(28), 17491-17497 (1998-07-04)
Insulin receptor substrates (IRSs) are tyrosine-phosphorylated following stimulation with insulin, insulin-like growth factors (IGFs), and interleukins. A key question is whether different IRSs play different roles to mediate insulin's metabolic and growth-promoting effects. In a novel system of insulin receptor-deficient
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Reduced phosphorylation of brain insulin receptor substrate and Akt proteins in apolipoprotein-E4 targeted replacement mice.
Ong, QR; Chan, ES; Lim, ML; Cole, GM; Wong, BS
Scientific Reports null
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