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Merck

914916

Sigma-Aldrich

Biotin-SS-tyramide

≥95%

Synonym(s):

N-(2-((3-(4-Hydroxyphenethylamino)-3-oxopropyl)disulfanyl)ethyl)-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, Cleavable biotin-phenol, Cleavable biotin-tyramide linker

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About This Item

Empirical Formula (Hill Notation):
C23H34N4O4S3
Molecular Weight:
526.74
UNSPSC Code:
12352200
NACRES:
NA.22

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Quality Level

Assay

≥95%

form

powder

storage temp.

−20°C

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This Item
913863914924914460
assay

≥95%

assay

-

assay

≥95%

assay

≥95%

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Application

Biotin-SS-tyramide is a cleavable, biotinylated reagent for tyramide signal amplification used in many applications including immunohistochemistry, in situ hybridization, electron microscopy, ELISA, and others. It can be used together with both chromogenic and fluorescence visualization methods. The linker can also be used for synthesis of chemical biology tools for labeling target proteins in biological experiments and assays. It possesses dual functionality: enrichment via the biotin and an embedded disulfide bridge for cleavage under reducing conditions or by glutathione in the lysosome.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Questions

  1. How to make stock solution of this product?

    1 answer
    1. The solubility and solution stability of this material have not been evaluated. External sources indicate that this compound may be prepared in DMSO at 10 mM and solutions may be stored at -20°C for up to 1 month or -80°C for up to 6 months. This information has not been validated.

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